Generator

Part:BBa_K2796059:Design

Designed by: Tuoyu Zhou   Group: iGEM18_LZU-CHINA   (2018-10-07)


HRE-Exosome booster-WPRE-SV40 poly(A) signal


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 482
    Illegal BglII site found at 2067
    Illegal XhoI site found at 5
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4685
    Illegal AgeI site found at 2947
    Illegal AgeI site found at 3567
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1657


Design Notes

We need follow RFC 10 rules.


Source

NCBI

References